| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cronin, Emma (13)
|
MRHAUP |
2:37.89 |
2:32.27 |
-5.62 |
| 2 |
Nguyen, Chloe T (13)
|
MRSSC |
2:33.60 |
2:33.36 |
-0.24 |
| 3 |
Garra, Isabella (14)
|
MRHYB |
2:33.07 |
2:33.87 |
0.80 |
| 4 |
Singh, Chloe M (14)
|
MRSSC |
2:45.90 |
2:34.01 |
-11.89 |
| 5 |
Fagundes, Eva (13)
|
MRHAUP |
2:50.39 |
2:34.95 |
-15.44 |
| 6 |
Rizzo, Vanessa R (13)
|
MREHY |
2:38.24 |
2:38.62 |
0.38 |
| 7 |
Masem, Madelyn M (14)
|
MRSSC |
2:46.20 |
2:41.70 |
-4.50 |
| 8 |
Mucha, Gianna (14)
|
MRHAUP |
2:49.57 |
2:42.28 |
-7.29 |
| 9 |
Acosta, Isabella (14)
|
MRHAUP |
2:50.76 |
2:42.69 |
-8.07 |
| 10 |
Norbury, Charlotte (13)
|
MRENAC |
2:45.33 |
2:44.36 |
-0.97 |
| 11 |
Bascon, Reese S (13)
|
MRHAUP |
2:45.02 |
2:46.37 |
1.35 |
| 12 |
Knopf, Sena (14)
|
MRHYB |
2:58.39 |
2:47.93 |
-10.46 |
| 13 |
O'Brien, Rachel S (13)
|
MRSSC |
2:44.66 |
2:48.32 |
3.66 |
| 14 |
Farez, Allison J (13)
|
MREHY |
2:50.92 |
2:48.48 |
-2.44 |
| 15 |
Menard, Madelyn X (14)
|
MRCIY |
2:25.29 |
2:49.32 |
24.03 |
| 16 |
Stratton, Charlotte S (13)
|
MRHYB |
3:03.10 |
2:50.94 |
-12.16 |
| 17 |
Vinci, Clare B (14)
|
MRSSC |
2:56.89 |
2:53.53 |
-3.36 |
| 18 |
Menard, Sophia E (13)
|
MRCIY |
|
2:55.21 |
- |
| 19 |
Ostuni, Lia P (13)
|
MRHYB |
3:12.96 |
2:56.08 |
-16.88 |
| 20 |
Mrose, Riley L (13)
|
MRSSC |
3:06.44 |
2:56.16 |
-10.28 |
| 21 |
Proctor, Campbell J (13)
|
MRHYB |
3:00.00 |
2:56.63 |
-3.37 |
| 22 |
Sirianni, Isabella V (14)
|
MRSSC |
3:12.30 |
2:59.94 |
-12.36 |
| 23 |
Haas-Colwell, Eleanor T (13)
|
MRSSC |
3:32.48 |
3:08.73 |
-23.75 |
| 24 |
Bruccoleri, Lucia A (13)
|
MRSSC |
|
3:09.66 |
- |
| 25 |
Erickson, Abigail G (13)
|
MRSSC |
|
3:12.24 |
- |
| 26 |
Britton, Sameera (13)
|
MRLIFE |
|
3:14.14 |
- |
|
Robertson, Olivia (13)
|
MREHY |
|
DQ |
- |